pCAGGS-S-501V2-FLAG
(Plasmid #177096)

• Purpose: 501V2 variant S-protein with c-terminal FLAG tag
• Depositing Lab: Daniel Conway
• Publication: Spike variants with FLAG tags (unpublished)

Backbone

• Vector backbone: pCAGGS
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: S-protein 501V2
• Species: SARS-CoV-2
• Entrez Gene: S (a.k.a. GU280_gp02, spike glycoprotein)
• Tag / Fusion Protein:
  • FLAG (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (unknown if destroyed)
• 3′ cloning site: BstEII (unknown if destroyed)
• 5′ sequencing primer: pCAG-F
• 3′ sequencing primer: Bglob-PA-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Plasmid #170449 was cut with EcoRI, and the second (c-terminal) BstEII site. This insert was removed and put into plasmid #156421 at EcoRI/BstEII sites, to create a full length B.1.1.7 spike protein with c-terminal FLAG.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA