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Addgene

pCAGGS-S-P1-FLAG
(Plasmid #177095)

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Item Catalog # Description Quantity Price (USD)
Plasmid 177095 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pCAGGS
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    S-protein P1
  • Species
    SARS-CoV-2
  • Entrez Gene
    S (a.k.a. GU280_gp02, spike glycoprotein)
  • Tag / Fusion Protein
    • FLAG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site BstEII (unknown if destroyed)
  • 5′ sequencing primer pCAG-F
  • 3′ sequencing primer Bglob-PA-R
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Plasmid #170450 was cut with EcoRI, and the second (c-terminal) BstEII site. This insert was removed and put into plasmid #156421 at EcoRI/BstEII sites, to create a full length B.1.1.7 spike protein with c-terminal FLAG.

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAGGS-S-P1-FLAG was a gift from Daniel Conway (Addgene plasmid # 177095 ; http://n2t.net/addgene:177095 ; RRID:Addgene_177095)
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