pHAGE-APPSw;T719N
(Plasmid #177094)

• Purpose: For lentiviral transduction of APPsw; T719N
• Depositing Lab: Wade Harper
• Publication: Park et al Nat Commun. 2022 Oct 16;13(1):6112. doi: 10.1038/s41467-022-33881-x.

Backbone

• Vector backbone: pHAGE
• Vector type: Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: APPsw;T719N
• Species: H. sapiens (human)
• Mutation: T719N, K651N and M652L (please see depositor comments)
• Entrez Gene: APP (a.k.a. AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, alpha-sAPP, preA4)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note there are two additional mutations in the insert compared to NCBI reference- K651N and M652L. Depositor confirms these mutations are not a concern for plasmid function.

Please visit https://www.biorxiv.org/content/10.1101/2022.03.31.486371v1 for bioRxiv preprint.