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Addgene

pHAGE-APPSw;T719N
(Plasmid #177094)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 177094 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHAGE
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    APPsw;T719N
  • Species
    H. sapiens (human)
  • Mutation
    T719N, K651N and M652L (please see depositor comments)
  • Entrez Gene
    APP (a.k.a. AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, alpha-sAPP, preA4)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note there are two additional mutations in the insert compared to NCBI reference- K651N and M652L. Depositor confirms these mutations are not a concern for plasmid function.

Please visit https://www.biorxiv.org/content/10.1101/2022.03.31.486371v1 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHAGE-APPSw;T719N was a gift from Wade Harper (Addgene plasmid # 177094 ; http://n2t.net/addgene:177094 ; RRID:Addgene_177094)

  • For your References section:

    Spatial snapshots of amyloid precursor protein intramembrane processing via early endosome proteomics. Park H, Hundley FV, Yu Q, Overmyer KA, Brademan DR, Serrano L, Paulo JA, Paoli JC, Swarup S, Coon JJ, Gygi SP, Wade Harper J. Nat Commun. 2022 Oct 16;13(1):6112. doi: 10.1038/s41467-022-33881-x. 10.1038/s41467-022-33881-x PubMed 36245040
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