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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 17709 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1/CT-GFP-TOPO
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 6150
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namerent2
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SpeciesH. sapiens (human)
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Insert Size (bp)3860
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Entrez GeneUPF2 (a.k.a. HUPF2, RENT2, smg-3)
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Tag
/ Fusion Protein
- GFP (C terminal on backbone)
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
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Addgene Notes
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
RNT2-GFP was a gift from Hal Dietz (Addgene plasmid # 17709 ; http://n2t.net/addgene:17709 ; RRID:Addgene_17709) -
For your References section:
Novel Upf2p orthologues suggest a functional link between translation initiation and nonsense surveillance complexes. Mendell JT, Medghalchi SM, Lake RG, Noensie EN, Dietz HC. Mol Cell Biol. 2000 Dec . 20(23):8944-57. 10.1128/MCB.20.23.8944-8957.2000 PubMed 11073994