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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 17706 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonena
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGLASTp
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Alt nameGlutamate-aspartate transporter promoter
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Alt nameSLC1A3
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SpeciesH. sapiens (human)
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Entrez GeneSLC1A3 (a.k.a. EA6, EAAT1, GLAST, GLAST1)
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Tag
/ Fusion Protein
- DsRed2 (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer na
- 3′ sequencing primer DsRed1-N (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GLASTp-DsRed2 was a gift from Nicholas Gaiano (Addgene plasmid # 17706 ; http://n2t.net/addgene:17706 ; RRID:Addgene_17706) -
For your References section:
Differential Notch signalling distinguishes neural stem cells from intermediate progenitors. Mizutani K, Yoon K, Dang L, Tokunaga A, Gaiano N. Nature. 2007 Sep 20. 449(7160):351-5. 10.1038/nature06090 PubMed 17721509