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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 17704 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCLE
- Backbone size w/o insert (bp) 7600
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Vector typeMammalian Expression, Retroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNIC
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Alt nameNotch intracellular domain
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Alt nameNotch
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SpeciesM. musculus (mouse)
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Insert Size (bp)1380
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Entrez GeneNotch1 (a.k.a. 9930111A19Rik, Mis6, N1, Tan1, lin-12)
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Tag
/ Fusion Protein
- Myc (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer na (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The portion of mouse Notch1 (NIC) used spans residues 1753–2185, and has a Myc tag at the N terminus.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCLEN was a gift from Nicholas Gaiano (Addgene plasmid # 17704 ; http://n2t.net/addgene:17704 ; RRID:Addgene_17704) -
For your References section:
Radial glial identity is promoted by Notch1 signaling in the murine forebrain. Gaiano N, Nye JS, Fishell G. Neuron. 2000 May . 26(2):395-404. 10.1016/S0896-6273(00)81172-1 PubMed 10839358
Map uploaded by the depositor.
