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pNTPTP alpha (Y789F)
(Plasmid #17702)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17702 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTet-splice
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5178
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    PTPalpha Y789F
  • Alt name
    receptor protein tyrosine phosphatase alpha
  • Alt name
    RPTPalpha
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2379
  • Mutation
    Y789F
  • Entrez Gene
    PTPRA (a.k.a. HEPTP, HLPR, HPTPA, HPTPalpha, LRP, PTPA, PTPRL2, R-PTP-alpha, RPTPA)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (unknown if destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)
  • 5′ sequencing primer LNCX
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See article for cloning details. The plasmid contains a CMV promoter under control of regulatory sequences from the tet operon such that, when cotransfected with the transactivator pTet-tTAk, it expresses protein only in the absence of doxycycline.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNTPTP alpha (Y789F) was a gift from Xinmin Zheng (Addgene plasmid # 17702 ; http://n2t.net/addgene:17702 ; RRID:Addgene_17702)

  • For your References section:

    A phosphotyrosine displacement mechanism for activation of Src by PTPalpha. Zheng XM, Resnick RJ, Shalloway D. EMBO J. 2000 Mar 1. 19(5):964-78. 10.1093/emboj/19.5.964 PubMed 10698938
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