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Addgene

pAAV-DIO-EF1a-TVA-P2A-EYFP
(Plasmid #177017)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 177017 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV2
  • Backbone size w/o insert (bp) 5777
  • Total vector size (bp) 7004
  • Vector type
    Mammalian Expression, AAV, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TVA-P2A-EYFP
  • Species
    Coturnix japonica
  • Insert Size (bp)
    1227
  • GenBank ID
    L22753.1
  • Entrez Gene
    CD320
  • Promoter CAGGS
  • Tag / Fusion Protein
    • EYFP (C terminal on insert)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer cctacagctcctgggcaacgtgc
  • 3′ sequencing primer CGCCACGTTGCCTGACAACGGGCC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-DIO-EF1a-TVA-P2A-EYFP was a gift from Peter Jonas (Addgene plasmid # 177017 ; http://n2t.net/addgene:177017 ; RRID:Addgene_177017)
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