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pLIB-HIS-TEV-Cyclin-B
(Plasmid #177012)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 177012 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLIB-HIS-TEV
  • Backbone size w/o insert (bp) 5022
  • Total vector size (bp) 6324
  • Vector type
    Insect Expression
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Cyclin B1
  • Alt name
    CCNB1
  • Species
    H. sapiens (human)
  • Mutation
    codon-optimised for insect cell expression
  • GenBank ID
    NP_114172.1 P14635-1
  • Entrez Gene
    CCNB1 (a.k.a. CCNB)
  • Tag / Fusion Protein
    • Polyhistidine-TEV (N terminal on backbone)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLIB-HIS-TEV-Cyclin-B was a gift from Andrea Musacchio (Addgene plasmid # 177012 ; http://n2t.net/addgene:177012 ; RRID:Addgene_177012)

  • For your References section:

    Reconstitution and use of highly active human CDK1:Cyclin-B:CKS1 complexes. Huis In 't Veld PJ, Wohlgemuth S, Koerner C, Muller F, Janning P, Musacchio A. Protein Sci. 2021 Nov 17. doi: 10.1002/pro.4233. 10.1002/pro.4233 PubMed 34791727
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