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Addgene

pYESir2HY-puro
(Plasmid #1770)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 1770 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBABE-puro
  • Backbone size w/o insert (bp) 5169
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Sir2
  • Alt name
    silent information regulator
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2500
  • Mutation
    H363Y, residue 363 changed from histidine to tyrosine, dominant negative
  • Entrez Gene
    SIRT1 (a.k.a. SIR2, SIR2L1, SIR2alpha)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SnaBI (not destroyed)
  • 5′ sequencing primer pBABE 5'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

SspI site in Amp gene.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYESir2HY-puro was a gift from Bob Weinberg (Addgene plasmid # 1770 ; http://n2t.net/addgene:1770 ; RRID:Addgene_1770)

  • For your References section:

    hSIR2(SIRT1) functions as an NAD-dependent p53 deacetylase. Vaziri H, Dessain SK, Ng Eaton E, Imai SI, Frye RA, Pandita TK, Guarente L, Weinberg RA. Cell 2001 Oct 19;107(2):149-59. 10.1016/S0092-8674(01)00527-X PubMed 11672523
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