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MIS18A_pLENTI-CAG-IRES-GFP
(Plasmid #176996)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 176996 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    plenti-CAG-gate-FLAG-IRES-GFP
  • Backbone manufacturer
    Derived from Addgene #107398; KanR replaced with AmpR
  • Backbone size w/o insert (bp) 9341
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    MIS18A
  • Alt name
    C21orf45
  • Alt name
    C21orf46
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    711
  • Entrez Gene
    MIS18A (a.k.a. B28, C21orf45, C21orf46, FASP1, MIS18alpha, hMis18alpha)
  • Promoter CAG

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Derived from Addgene #53821

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MIS18A_pLENTI-CAG-IRES-GFP was a gift from Roger Reeves (Addgene plasmid # 176996 ; http://n2t.net/addgene:176996 ; RRID:Addgene_176996)

  • For your References section:

    Overexpression screen of chromosome 21 genes reveals modulators of Sonic hedgehog signaling relevant to Down syndrome. Moyer AJ, Fernandez FX, Li Y, Klinedinst DK, Florea LD, Kazuki Y, Oshimura M, Reeves RH. Dis Model Mech. 2023 Apr 1;16(4):dmm049712. doi: 10.1242/dmm.049712. Epub 2023 Apr 13. 10.1242/dmm.049712 PubMed 36995257
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