Sufu_pLENTI-CAG-IRES-GFP
(Plasmid
#176993)
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PurposeMammalian lentiviral expression vector encoding Sufu
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 176993 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneplenti-CAG-gate-FLAG-IRES-GFP
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Backbone manufacturerDerived from Addgene #107398; KanR replaced with AmpR
- Backbone size w/o insert (bp) 9341
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameSufu
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Alt nameb2b273C
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SpeciesM. musculus (mouse)
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Insert Size (bp)1518
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Entrez GeneSufu (a.k.a. Su(fu))
- Promoter CAG
Cloning Information
- Cloning method TOPO Cloning
Resource Information
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A portion of this plasmid was derived from a plasmid made byDerived from Addgene #65431
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Sufu_pLENTI-CAG-IRES-GFP was a gift from Roger Reeves (Addgene plasmid # 176993 ; http://n2t.net/addgene:176993 ; RRID:Addgene_176993) -
For your References section:
Overexpression screen of chromosome 21 genes reveals modulators of Sonic hedgehog signaling relevant to Down syndrome. Moyer AJ, Fernandez FX, Li Y, Klinedinst DK, Florea LD, Kazuki Y, Oshimura M, Reeves RH. Dis Model Mech. 2023 Apr 1;16(4):dmm049712. doi: 10.1242/dmm.049712. Epub 2023 Apr 13. 10.1242/dmm.049712 PubMed 36995257
