DsRed2_pcDNA6.2/EmGFP-Bsd
(Plasmid
#176988)
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PurposeMammalian expression vector encoding DsRed2 and EmGFP-Bsd
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 176988 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA6.2/EmGFP-Bsd/V5-DEST
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Backbone manufacturerInvitrogen (V36620)
- Backbone size w/o insert (bp) 5983
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Vector typeMammalian Expression
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Selectable markersEmGFP fused to Blasticidin (bsd)-resistance marker
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDsRed2
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SpeciesDiscosoma sp
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Insert Size (bp)749
- Promoter CMV
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer V5 reverse (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byDerived from Addgene #22523
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
DsRed2 expressed under the control of CMV promoter and EmGFP-Bsd under the control of PGK
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
DsRed2_pcDNA6.2/EmGFP-Bsd was a gift from Roger Reeves (Addgene plasmid # 176988 ; http://n2t.net/addgene:176988 ; RRID:Addgene_176988) -
For your References section:
Overexpression screen of chromosome 21 genes reveals modulators of Sonic hedgehog signaling relevant to Down syndrome. Moyer AJ, Fernandez FX, Li Y, Klinedinst DK, Florea LD, Kazuki Y, Oshimura M, Reeves RH. Dis Model Mech. 2023 Apr 1;16(4):dmm049712. doi: 10.1242/dmm.049712. Epub 2023 Apr 13. 10.1242/dmm.049712 PubMed 36995257
