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Addgene

MIS18A_pcDNA6.2/EmGFP-Bsd
(Plasmid #176973)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 176973 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA6.2/EmGFP-Bsd/V5-DEST
  • Backbone manufacturer
    Invitrogen (V36620)
  • Backbone size w/o insert (bp) 5983
  • Vector type
    Mammalian Expression
  • Selectable markers
    EmGFP fused to Blasticidin (bsd)-resistance marker

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MIS18A
  • Alt name
    C21orf45
  • Alt name
    C21orf46
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    702
  • Entrez Gene
    MIS18A (a.k.a. B28, C21orf45, C21orf46, FASP1, MIS18alpha, hMis18alpha)
  • Promoter CMV

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Derived from Addgene #53821

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

MIS18A (NM_018944) expressed under the control of CMV promoter and EmGFP-Bsd under the control of PGK

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MIS18A_pcDNA6.2/EmGFP-Bsd was a gift from Roger Reeves (Addgene plasmid # 176973 ; http://n2t.net/addgene:176973 ; RRID:Addgene_176973)

  • For your References section:

    Overexpression screen of chromosome 21 genes reveals modulators of Sonic hedgehog signaling relevant to Down syndrome. Moyer AJ, Fernandez FX, Li Y, Klinedinst DK, Florea LD, Kazuki Y, Oshimura M, Reeves RH. Dis Model Mech. 2023 Apr 1;16(4):dmm049712. doi: 10.1242/dmm.049712. Epub 2023 Apr 13. 10.1242/dmm.049712 PubMed 36995257
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