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Addgene

pcDNA3.1(+)_Granulin 7+linker8
(Plasmid #176930)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 176930 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1(+)
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Granulin 7+linker8
  • Alt name
    GRN
  • Species
    H. sapiens (human)
  • Entrez Gene
    GRN (a.k.a. CLN11, FTD2, GEP, GP88, PCDGF, PEPI, PGRN)
  • Tag / Fusion Protein
    • Twin-Strep and FLAG tags encoded after Signal Peptide (N terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1(+)_Granulin 7+linker8 was a gift from Thomas Kukar (Addgene plasmid # 176930 ; http://n2t.net/addgene:176930 ; RRID:Addgene_176930)

  • For your References section:

    Intracellular Proteolysis of Progranulin Generates Stable, Lysosomal Granulins that Are Haploinsufficient in Patients with Frontotemporal Dementia Caused by GRN Mutations. Holler CJ, Taylor G, Deng Q, Kukar T. eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-0100-17. doi: 10.1523/ENEURO.0100-17.2017. eCollection 2017 Jul-Aug. 10.1523/ENEURO.0100-17.2017 PubMed 28828399
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