pTPTP alpha
(Plasmid #17691)

• Depositing Lab: Xinmin Zheng
• Publication: Zheng et al EMBO J. 2000 Mar 1. 19(5):964-78.

Backbone

• Vector backbone: pTet-splice
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5178
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: PTPalpha
• Alt name: receptor protein tyrosine phosphatase alpha
• Alt name: RPTPalpha
• Species: H. sapiens (human)
• Insert Size (bp): 2406
• Entrez Gene: PTPRA (a.k.a. HEPTP, HLPR, HPTPA, HPTPalpha, LRP, PTPA, PTPRL2, R-PTP-alpha, RPTPA)
• Tag / Fusion Protein:
  • HA (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (unknown if destroyed)
• 3′ cloning site: HindIII (unknown if destroyed)
• 5′ sequencing primer: LNCX

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

PTPalpha-HA was amplified from plasmid pXJ41PTPalpha using the 5' primer 5'-CGCCAAGCTTGGCCACCATGGATTCCTGGTTCATTCTTGT-3' and the 3' primer 5'-TGTCAAGCTTCATGCGTAGTCTGCACGTCGTATGGGTACTTGAAGT- TGGCATAATCTGA-3'. The 5' primer contains a HindIII site, a Kozak consensus sequence CCACC and the start codon. The 3' primer contains the coding sequence for the HA epitope YPYDVPDYA, the stop codon and a HindIII site. HindIII-cleaved PCR products were ligated into pTet-Splice. The plasmid contains a CMV promoter under control of regulatory sequences from the tet operon such that, when cotransfected with the transactivator pTet-tTAk (Life Technologies), it expresses protein only in the absence of doxycycline.