modRNAc0-p53DD
(Plasmid
#176902)
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PurposeIVT template plasmid for making p53DD modRNA
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 176902 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonemodRNA cap0 plasmid
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Vector typeIVT template plasmid
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namep53DD
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Alt nameDominant negative form of p53
- Promoter T7 promoter
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoR1 (not destroyed)
- 3′ cloning site Spe1 (not destroyed)
- 5′ sequencing primer T7 promoter
- 3′ sequencing primer M13 Rvs (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2022.05.24.493220v1 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
modRNAc0-p53DD was a gift from Xiaojun Lian (Addgene plasmid # 176902 ; http://n2t.net/addgene:176902 ; RRID:Addgene_176902) -
For your References section:
Robust genome editing via modRNA-based Cas9 or base editor in human pluripotent stem cells. Haideri T, Howells A, Jiang Y, Yang J, Bao X, Lian XL. Cell Rep Methods. 2022 Sep 7;2(9):100290. doi: 10.1016/j.crmeth.2022.100290. eCollection 2022 Sep 19. 10.1016/j.crmeth.2022.100290 PubMed 36160051
