mILK-L207W-EFGP
(Plasmid #176900)

• Purpose: mouse ILK with L207W point mutation cloned into pEGFP-N1
• Depositing Lab: Sara Wickström
• Publication: Martin et al Proc Natl Acad Sci U S A. 2022 Mar 15;119(11):e2106098119. doi: 10.1073/pnas.2106098119. Epub 2022 Mar 8.

Backbone

• Vector backbone: pEGFP-N1
• Backbone manufacturer: Clontech
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Integrin-linked kinase
• Alt name: Ilk
• Species: M. musculus (mouse)
• Insert Size (bp): 1370
• Mutation: Leucine 207 to Tryptophane
• GenBank ID: NM_001161724
• Entrez Gene: Ilk (a.k.a. ESTM24, ILK-1, ILK-2, p59ILK)
• Promoter: CNV
• Tag / Fusion Protein:
  • EGFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoR1 (not destroyed)
• 3′ cloning site: Age1 (not destroyed)
• 5′ sequencing primer: CGC AAA TGG GCG GTA GGC GTG
• 3′ sequencing primer: CGT CGC CGT CCA GCT CGA CCA G

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2021.03.30.437490v1 for bioRxiv preprint.