Skip to main content
Addgene

pcDNA3 HA Sam68 WT
(Plasmid #17690)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17690 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pcDNA3-HA
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Sam68
  • Alt name
    Sam68
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Khdrbs1 (a.k.a. Sam68, p62, p68)
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3 HA Sam68 WT was a gift from David Shalloway (Addgene plasmid # 17690 ; http://n2t.net/addgene:17690 ; RRID:Addgene_17690)

  • For your References section:

    Specificity and determinants of Sam68 RNA binding. Implications for the biological function of K homology domains. Lin Q, Taylor SJ, Shalloway D. J Biol Chem. 1997 Oct 24. 272(43):27274-80. 10.1074/jbc.272.43.27274 PubMed 9341174
Related items:
Commonly requested with: