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mILK-R349A-EFGP
(Plasmid #176898)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 176898 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Integrin-linked kinase
  • Alt name
    Ilk
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1370
  • Mutation
    Arginine 349 to Alanine
  • GenBank ID
    NM_001161724
  • Entrez Gene
    Ilk (a.k.a. ESTM24, ILK-1, ILK-2, p59ILK)
  • Promoter CNV
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site Age1 (not destroyed)
  • 5′ sequencing primer CGC AAA TGG GCG GTA GGC GTG
  • 3′ sequencing primer CGT CGC CGT CCA GCT CGA CCA G
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mILK-R349A-EFGP was a gift from Sara Wickström (Addgene plasmid # 176898 ; http://n2t.net/addgene:176898 ; RRID:Addgene_176898)

  • For your References section:

    ATP allosterically stabilizes integrin-linked kinase for efficient force generation. Martin IM, Nava MM, Wickstrom SA, Grater F. Proc Natl Acad Sci U S A. 2022 Mar 15;119(11):e2106098119. doi: 10.1073/pnas.2106098119. Epub 2022 Mar 8. 10.1073/pnas.2106098119 PubMed 35259013
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