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Addgene

pYPQ220-Ultra
(Plasmid #176889)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 176889 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pYPQ167
  • Total vector size (bp) 7214
  • Vector type
    Plant Expression, CRISPR ; Gateway compatible Cas12a entry clone

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AsCas12a
  • Species
    Acidaminococcus sp. BV3L6
  • Tags / Fusion Proteins
    • SV40 NLS (N terminal on insert)
    • Nucleoplasmin NLS (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYPQ220-Ultra was a gift from Yiping Qi (Addgene plasmid # 176889 ; http://n2t.net/addgene:176889 ; RRID:Addgene_176889)

  • For your References section:

    Highly Efficient Genome Editing in Plant Protoplasts by Ribonucleoprotein Delivery of CRISPR-Cas12a Nucleases. Zhang Y, Cheng Y, Fang H, Roberts N, Zhang L, Vakulskas CA, Niedz RP, Culver JN, Qi Y. Front Genome Ed. 2022 Jan 31;4:780238. doi: 10.3389/fgeed.2022.780238. eCollection 2022. 10.3389/fgeed.2022.780238 PubMed 35174354
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