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Addgene

pRN119
(Plasmid #176884)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 176884 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTRANS_221
  • Vector type
    Plant Expression
  • Selectable markers
    Kanamycin in plants

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CmYLCV:Luc+:HSP, Nos::WUS2:pinII, and 35:STM:35S
  • Species
    A. thaliana (mustard weed)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRN119 was a gift from Daniel Voytas (Addgene plasmid # 176884 ; http://n2t.net/addgene:176884 ; RRID:Addgene_176884)

  • For your References section:

    Direct delivery and fast-treated Agrobacterium co-culture (Fast-TrACC) plant transformation methods for Nicotiana benthamiana. Cody JP, Maher MF, Nasti RA, Starker CG, Chamness JC, Voytas DF. Nat Protoc. 2022 Oct 17. pii: 10.1038/s41596-022-00749-9. doi: 10.1038/s41596-022-00749-9. 10.1038/s41596-022-00749-9 PubMed 36253612
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