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Addgene

pJC225
(Plasmid #176883)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 176883 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMOD_B0000
  • Vector type
    cloning

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

MoClo compatible, allows transition from MoClo cloning system into the Cermak, et al., 2017 Golden Gate assembly system

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJC225 was a gift from Daniel Voytas (Addgene plasmid # 176883 ; http://n2t.net/addgene:176883 ; RRID:Addgene_176883)

  • For your References section:

    Direct delivery and fast-treated Agrobacterium co-culture (Fast-TrACC) plant transformation methods for Nicotiana benthamiana. Cody JP, Maher MF, Nasti RA, Starker CG, Chamness JC, Voytas DF. Nat Protoc. 2022 Oct 17. pii: 10.1038/s41596-022-00749-9. doi: 10.1038/s41596-022-00749-9. 10.1038/s41596-022-00749-9 PubMed 36253612
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