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Addgene

pcDNA3 HA Sam68 deltaKH
(Plasmid #17688)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17688 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3-HA
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Sam68
  • Species
    M. musculus (mouse)
  • Mutation
    aa 157-256 deleted (KH domain)
  • Entrez Gene
    Khdrbs1 (a.k.a. Sam68, p62, p68)
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pcDNA3 HA-Sam68 was generated by subcloning a BamHI/EcoRI insert from pGEX-Sam68 into pcDNA3-HA, which encodes amino-terminally hemagglutinin (HA) epitope-tagged proteins when cloned into its BamHI site.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3 HA Sam68 deltaKH was a gift from David Shalloway (Addgene plasmid # 17688 ; http://n2t.net/addgene:17688 ; RRID:Addgene_17688)

  • For your References section:

    Specificity and determinants of Sam68 RNA binding. Implications for the biological function of K homology domains. Lin Q, Taylor SJ, Shalloway D. J Biol Chem. 1997 Oct 24. 272(43):27274-80. 10.1074/jbc.272.43.27274 PubMed 9341174
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