pGEX-2T Sam68
(Plasmid
#17687)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 17687 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEX2T
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Backbone manufacturerAmersham Pharmacia
- Backbone size w/o insert (bp) 5000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSam68
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SpeciesM. musculus (mouse)
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Entrez GeneKhdrbs1 (a.k.a. Sam68, p62, p68)
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site EcoRI (unknown if destroyed)
- 5′ sequencing primer pGEX 5' (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
For additional information regarding the use of this plasmid, please see: Lin et al., J Biol Chem. 1997 Oct 24;272(43):27274-80. https://www.ncbi.nlm.nih.gov/pubmed/9341174
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX-2T Sam68 was a gift from David Shalloway (Addgene plasmid # 17687 ; http://n2t.net/addgene:17687 ; RRID:Addgene_17687) -
For your References section:
Phosphorylation of the Src substrate Sam68 by Cdc2 during mitosis. Resnick RJ, Taylor SJ, Lin Q, Shalloway D. Oncogene. 1997 Sep;15(11):1247-53. 10.1038/sj.onc.1201289 PubMed 9315091
