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Addgene

pTAG2E
(Plasmid #176865)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 176865 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAGM9121
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sgRNA_2E
  • Alt name
    LacZ
  • gRNA/shRNA sequence
    unknown
  • Species
    Synthetic
  • Mutation
    WT

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

MoClo compatible

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTAG2E was a gift from Daniel Voytas (Addgene plasmid # 176865 ; http://n2t.net/addgene:176865 ; RRID:Addgene_176865)

  • For your References section:

    Direct delivery and fast-treated Agrobacterium co-culture (Fast-TrACC) plant transformation methods for Nicotiana benthamiana. Cody JP, Maher MF, Nasti RA, Starker CG, Chamness JC, Voytas DF. Nat Protoc. 2022 Oct 17. pii: 10.1038/s41596-022-00749-9. doi: 10.1038/s41596-022-00749-9. 10.1038/s41596-022-00749-9 PubMed 36253612
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