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Addgene

UBQLN2_450-624W
(Plasmid #176852)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 176852 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET24b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5309
  • Total vector size (bp) 5768
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    UBQLN2_450C_W
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    534
  • Mutation
    deletion of residues 1-449 and addition of C-terminal W
  • GenBank ID
    NM_013444
  • Entrez Gene
    UBQLN2 (a.k.a. ALS15, CHAP1, DSK2, HRIHFB2157, N4BP4, PLIC2)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 term
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Addgene plasmid #8661 (deposited by Peter Howley)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    UBQLN2_450-624W was a gift from Carlos Castañeda (Addgene plasmid # 176852 ; http://n2t.net/addgene:176852 ; RRID:Addgene_176852)

  • For your References section:

    Ubiquitin Modulates Liquid-Liquid Phase Separation of UBQLN2 via Disruption of Multivalent Interactions. Dao TP, Kolaitis RM, Kim HJ, O'Donovan K, Martyniak B, Colicino E, Hehnly H, Taylor JP, Castaneda CA. Mol Cell. 2018 Mar 15;69(6):965-978.e6. doi: 10.1016/j.molcel.2018.02.004. Epub 2018 Mar 8. 10.1016/j.molcel.2018.02.004 PubMed 29526694
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