pLV-hPLXNB2_PGK_Neo
(Plasmid
#176848)
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Purposelentiviral vector for constitutive PLXNB2 overexpression
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 176848 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLENTI PGK Neo DEST
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Backbone manufacturerEric Campeau & Paul Kaufman (Addgene plasmid # 19067)
- Backbone size w/o insert (bp) 9778
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Vector typeLentiviral
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehPLXNB2
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SpeciesH. sapiens (human)
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Insert Size (bp)5517
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Mutationfull length PLXNB2
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Entrez GenePLXNB2 (a.k.a. MM1, Nbla00445, PLEXB2, dJ402G11.3)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer PGK-F (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This is the wild-type full length cDNA sequence for PLXNB2. This plasmid is similar to Addgene plasmid # 86237, but is not CRISPR-resistant.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLV-hPLXNB2_PGK_Neo was a gift from Roland Friedel (Addgene plasmid # 176848 ; http://n2t.net/addgene:176848 ; RRID:Addgene_176848) -
For your References section:
Plexin-B2 orchestrates collective stem cell dynamics via actomyosin contractility, cytoskeletal tension and adhesion. Junqueira Alves C, Dariolli R, Haydak J, Kang S, Hannah T, Wiener RJ, DeFronzo S, Tejero R, Gusella GL, Ramakrishnan A, Alves Dias R, Wojcinski A, Kesari S, Shen L, Sobie EA, Rodrigues Furtado de Mendonca JP, Azeloglu EU, Zou H, Friedel RH. Nat Commun. 2021 Oct 14;12(1):6019. doi: 10.1038/s41467-021-26296-7. 10.1038/s41467-021-26296-7 PubMed 34650052