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Addgene

pEE071
(Plasmid #176828)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 176828 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET21b(+)
  • Backbone manufacturer
    EMD Biosciences
  • Backbone size w/o insert (bp) 5366
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    710
  • Alt name
    Thh_Est
  • Alt name
    Thermobifida halotolerans AFA45122.1
  • Species
    Thermobifida halotolerans
  • Insert Size (bp)
    783
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer T7 For
  • 3′ sequencing primer T7 Rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Accession Number AFA45122.1

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEE071 was a gift from Gregg Beckham (Addgene plasmid # 176828 ; http://n2t.net/addgene:176828 ; RRID:Addgene_176828)

  • For your References section:

    Sourcing thermotolerant poly(ethylene terephthalate) hydrolase scaffolds from natural diversity. Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, Beckham GT, McGeehan JE. Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x. 10.1038/s41467-022-35237-x PubMed 36543766
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