pEE055
(Plasmid
#176812)
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PurposeEncodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 176812 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET21b(+)
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Backbone manufacturerEMD Biosciences
- Backbone size w/o insert (bp) 5366
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name606
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Alt nameMarinactinospora thermotolerans WP_078759821.1
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SpeciesMarinactinospora thermotolerans
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Insert Size (bp)786
- Promoter T7
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Tag
/ Fusion Protein
- 6xHis (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (unknown if destroyed)
- 3′ cloning site XhoI (unknown if destroyed)
- 5′ sequencing primer T7 For
- 3′ sequencing primer T7 Rev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Accession Number WP_078759821.1
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEE055 was a gift from Gregg Beckham (Addgene plasmid # 176812 ; http://n2t.net/addgene:176812 ; RRID:Addgene_176812) -
For your References section:
Sourcing thermotolerant poly(ethylene terephthalate) hydrolase scaffolds from natural diversity. Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, Beckham GT, McGeehan JE. Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x. 10.1038/s41467-022-35237-x PubMed 36543766