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Addgene

pcSrc416/527
(Plasmid #17677)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17677 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEVX
  • Backbone size w/o insert (bp) 6400
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    c-src Y416F Y527F
  • Alt name
    c-Src
  • Alt name
    src
  • Species
    G. gallus (chicken)
  • Mutation
    Tyr 416 changed to Phe . Tyr 527 changed to Phe .
  • Entrez Gene
    SRC (a.k.a. PP60C-SCR, SDR, c-src)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Based on plasmid pM5HHB5.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcSrc416/527 was a gift from David Shalloway (Addgene plasmid # 17677 ; http://n2t.net/addgene:17677 ; RRID:Addgene_17677)

  • For your References section:

    Activation and suppression of pp60c-src transforming ability by mutation of its primary sites of tyrosine phosphorylation. Kmiecik TE, Shalloway D. Cell. 1987 Apr 10. 49(1):65-73. 10.1016/0092-8674(87)90756-2 PubMed 3103925
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