pcSrc527
(Plasmid #17675)

• Depositing Lab: David Shalloway
• Publication: Kmiecik et al Cell. 1987 Apr 10. 49(1):65-73.

Backbone

• Vector backbone: pEVX
• Backbone size w/o insert (bp): 6400
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: c-src Y527F
• Alt name: c-Src
• Alt name: src
• Species: G. gallus (chicken)
• Mutation: Tyr 527 changed to Phe . Activates transforming and kinase activities.
• Entrez Gene: SRC (a.k.a. PP60C-SCR, SDR, c-src)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: n/a

Resource Information

• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Based on plasmid pM5HHB5.