pMcSrc
(Plasmid #17672)

• Depositing Lab: David Shalloway
• Publication: Johnson et al Mol Cell Biol. 1985 May . 5(5):1073-83.

Backbone

• Vector backbone: pEVX
• Backbone size w/o insert (bp): 6400
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: c-src
• Alt name: c-Src
• Alt name: src
• Species: G. gallus (chicken)
• Insert Size (bp): 8100
• Entrez Gene: SRC (a.k.a. PP60C-SCR, SDR, c-src)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI / BglII (destroyed during cloning)
• 3′ cloning site: BamHI / BglII (destroyed during cloning)
• 5′ sequencing primer: n/a

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pMcsrc was constructed by gel purifying the 8.1-kilobase (kb) BamHI c-src fragment running from c-src coordinates -2.1 to 6.0 kb and ligating it into plasmid pEVX which had been linearized at a unique BglII site lying between the two Moloney murine leukemia virus (MoMLV) long terminal
repeats (LTRs). The upstream BamHI site is about 270 base
pairs (bp) upstream from the beginning of c-src exon no. 2, whereas the downstream site lies about 1.2 kb beyond the c-src termination codon.