pBABE-neo
(Plasmid #1767)

• Purpose: (Empty Backbone) Retroviral vector for cloning and expressing your gene of interest. Neomycin selection. This plasmid is not available at Addgene.
• Depositing Labs: Hartmut Land, Jay Morgenstern
• Publication: Morgenstern et al Nucleic Acids Res. 1990 Jun 25;18(12):3587-96.

Backbone

• Vector backbone: pBABE-neo
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: pBABE 5'
• 3′ sequencing primer: pBABE 3'

Resource Information

• Supplemental Documents:
  • pBABE protocol
• Articles Citing this Plasmid:
  • 20 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please acknowledge Jay Morgenstern and Hartmut Land and cite the following article if you use this plasmid in a publication:
Morgenstern JP, Land H., 1990, Nucleic Acids Research 18(12):3587-96.

Note: There is an extra ~300 bp of vector sequence between the HindIII site and the neomycin gene that is not depicted in the author's sequence.

If you are using the pBABE protocol from the Weinberg Lab to generate virus, please note that the Weinberg Lab recommends using pUMVC (Addgene #8449) and VSV-G (#8454) for packaging. The pCL-Eco plasmid listed in their protocol should be substituted with pUMVC.