Skip to main content
Addgene

pcDNA6.2-YFP-CWT
(Plasmid #176689)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 176689 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pcDNA6.2
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cell wall-targeting domain of Lysostaphin
  • Alt name
    CWT
  • Species
    Aequorea victoria (modified), Staphylococcus simulans
  • Insert Size (bp)
    719
  • Mutation
    deletion of protase domain of Lysostaphin
  • Promoter CMV
  • Tag / Fusion Protein
    • YFP (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA6.2-YFP-CWT was a gift from Martin Fraunholz (Addgene plasmid # 176689 ; http://n2t.net/addgene:176689 ; RRID:Addgene_176689)

  • For your References section:

    Staphylococcus aureus Exploits a Non-ribosomal Cyclic Dipeptide to Modulate Survival within Epithelial Cells and Phagocytes. Blattner S, Das S, Paprotka K, Eilers U, Krischke M, Kretschmer D, Remmele CW, Dittrich M, Muller T, Schuelein-Voelk C, Hertlein T, Mueller MJ, Huettel B, Reinhardt R, Ohlsen K, Rudel T, Fraunholz MJ. PLoS Pathog. 2016 Sep 15;12(9):e1005857. doi: 10.1371/journal.ppat.1005857. eCollection 2016 Sep. 10.1371/journal.ppat.1005857 PubMed 27632173
Related items:
Commonly requested with: