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Addgene

pAaePUb::Cas9-2A-Neo
(Plasmid #176680)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 176680 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19
  • Vector type
    Insect Expression ; CRISPR
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    spCas9; NeoR (aminoglycoside phosphotransferase from Tn5)
  • Alt name
    Drosophila codon optimized spCas9
  • Species
    Ae. Aegypti, Streptococcus pyogenes
  • Mutation
    Drosophila codon optimized
  • Promoter Ae. aegypti poly-ubiquitin (AAEL003888)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAaePUb::Cas9-2A-Neo was a gift from Norbert Perrimon (Addgene plasmid # 176680 ; http://n2t.net/addgene:176680 ; RRID:Addgene_176680)

  • For your References section:

    Bioinformatic and cell-based tools for pooled CRISPR knockout screening in mosquitos. Viswanatha R, Mameli E, Rodiger J, Merckaert P, Feitosa-Suntheimer F, Colpitts TM, Mohr SE, Hu Y, Perrimon N. Nat Commun. 2021 Nov 24;12(1):6825. doi: 10.1038/s41467-021-27129-3. 10.1038/s41467-021-27129-3 PubMed 34819517
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