pEVX
(Plasmid
#17666)
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Purpose(Empty Backbone)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 17666 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEVX
- Backbone size (bp) 6400
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Vector typeMammalian Expression, Retroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNone
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
In the Author's Map, plasmid pEVX contains the
EVX proviral DNA (outlined), flanking cellular DNA (bold black line), and
pBR322 DNA (narrow black line). The LTR's are Moloney murine leukemia virus (MoMLV) long terminal repeats (LTRs).
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEVX was a gift from Michael Botchan (Addgene plasmid # 17666 ; http://n2t.net/addgene:17666 ; RRID:Addgene_17666) -
For your References section:
Transformation mediated by the SV40 T antigens: separation of the overlapping SV40 early genes with a retroviral vector. Kriegler M, Perez CF, Hardy C, Botchan M. Cell. 1984 Sep . 38(2):483-91. 10.1016/0092-8674(84)90503-8 PubMed 6088084
Map uploaded by the depositor.
