pCDH-EF1-mVenus-p27K−
(Plasmid #176651)

• Purpose: It encodes an mVenus fused to a mutant p27K-, which lacks binding affinity to Cdk. This reporter helps to identify quiescent disseminated tumor cells (in G0 phase of cell cycle).
• Depositing Lab: Mohamed Bentires-Alj
• Publication: Correia et al Nature. 2021 Jun;594(7864):566-571. doi: 10.1038/s41586-021-03614-z. Epub 2021 Jun 2.

Backbone

• Vector backbone: pCDH
• Backbone manufacturer: System Biosciences
• Backbone size w/o insert (bp): 7082
• Total vector size (bp): 8420
• Vector type: Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mVenus-p27K-
• Alt name: Cdkn1b
• Species: M. musculus (mouse)
• Insert Size (bp): 1338
• Mutation: Mutant K- (lacks affinity to Cdk)
• Entrez Gene: Cdkn1b (a.k.a. Kip1, p27, p27Kip1)
• Tag / Fusion Protein:
  • mVenus (N terminal on backbone)

Resource Information

• Supplemental Documents:
  • pcdh-ef1-p27-t2a-puro.gb
• A portion of this plasmid was derived from a plasmid made by: Toshihiko Oki and Toshio Kitamura, The University of Tokyo

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry