p43BgI
(Plasmid
#17665)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 17665 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonena
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCx43 3'UTR
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Alt nameCx43
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SpeciesM. musculus (mouse)
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Entrez GeneGja1 (a.k.a. Cnx43, Cx43, Cx43alpha1, Cxnk1, Gja-1, Npm1, connexin43)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
3'UTR of Mus musculus Gja1 (Cx43). For in situ riboprobe.
Anti-Sense: use SP6 and cut with ClaI.
Sense: use T7 and cut with HindIII.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p43BgI was a gift from Cecilia Lo (Addgene plasmid # 17665 ; http://n2t.net/addgene:17665 ; RRID:Addgene_17665)