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Addgene

pN1-mCherry-NKD2
(Plasmid #176641)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 176641 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pmCherryN1
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Homo sapiens NKD inhibitor of WNT signaling pathway 2
  • Alt name
    NKD2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1355
  • GenBank ID
    NM_033120.4
  • Entrez Gene
    NKD2 (a.k.a. Naked2)
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry tag (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pN1-mCherry-NKD2 was a gift from Sonal Srikanth (Addgene plasmid # 176641 ; http://n2t.net/addgene:176641 ; RRID:Addgene_176641)

  • For your References section:

    NKD2 mediates stimulation-dependent ORAI1 trafficking to augment Ca(2+) entry in T cells. Wu B, Woo JS, Vila P, Jew M, Leung J, Sun Z, Srikanth S, Gwack Y. Cell Rep. 2021 Aug 24;36(8):109603. doi: 10.1016/j.celrep.2021.109603. 10.1016/j.celrep.2021.109603 PubMed 34433025
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