pCMV3XFLAGm43CT
(Plasmid
#17664)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 17664 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonep3XFLAG-CMV-7.1?
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Backbone manufacturerSigma
- Backbone size w/o insert (bp) 3800
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCx43 CT
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Alt nameCx43
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SpeciesM. musculus (mouse)
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Insert Size (bp)450
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MutationCytoplasmic tail: aa 236-382
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GenBank IDU02451
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Entrez GeneGja1 (a.k.a. Cnx43, Cx43, Cx43alpha1, Cxnk1, Gja-1, Npm1, connexin43)
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Tag
/ Fusion Protein
- 3x Flag (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV3XFLAGm43CT was a gift from Cecilia Lo (Addgene plasmid # 17664 ; http://n2t.net/addgene:17664 ; RRID:Addgene_17664)
Map uploaded by the depositor.
