pCS2-BiNLS-fYap
(Plasmid #176623)

• Purpose: expresses optofYap in zebrafish
• Depositing Lab: Timothy Saunders
• Publication: Toh et al EMBO Rep. 2022 Sep 5;23(9):e54401. doi: 10.15252/embr.202154401. Epub 2022 Jul 25.

Backbone

• Vector backbone: pCS2+
• Backbone size w/o insert (bp): 4091
• Total vector size (bp): 6551
• Vector type: Zebrafish expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Yes associated protein
• Alt name: Yap
• Species: D. rerio (zebrafish)
• Insert Size (bp): 2706
• Entrez Gene: yap1 (a.k.a. YAP65, cb194, sb:cb194, si:ch211-181p1.5, si:dkey-3b8.3, wu:fc18c04, zYAP, zgc:158380)
• Promoter: SP6
• Tag / Fusion Protein:
  • mCherry (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (unknown if destroyed)
• 3′ cloning site: PspXI (unknown if destroyed)
• 5′ sequencing primer: ATTTAGGTGACACTATAGA
• 3′ sequencing primer: ACTATAGTGAGTCGTATTA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Vector backbone pDN34 is based on the LINuS method for optogenetic control of nuclear import.