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pCSJ982
(Plasmid #176622)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 176622 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJO629
  • Backbone manufacturer
    Alexander Varshavsky's lab
  • Backbone size w/o insert (bp) 8017
  • Total vector size (bp) 9328
  • Vector type
    Yeast Expression
  • Selectable markers
    HIS3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Ub-MPGLW-Fbp1
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1355
  • Mutation
    Changed first 4 N-terminal amino acid residues of Fbp1 to PGLW
  • Promoter TDH3tc3
  • Tag / Fusion Protein
    • Ubiquitin N-terminal fusion and triple flag tag C-terminal fusion

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AscI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer M13-forward
  • 3′ sequencing primer M13-reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCSJ982 was a gift from Alexander Varshavsky (Addgene plasmid # 176622 ; http://n2t.net/addgene:176622 ; RRID:Addgene_176622)

  • For your References section:

    Recognition of nonproline N-terminal residues by the Pro/N-degron pathway. Dong C, Chen SJ, Melnykov A, Weirich S, Sun K, Jeltsch A, Varshavsky A, Min J. Proc Natl Acad Sci U S A. 2020 Jun 23;117(25):14158-14167. doi: 10.1073/pnas.2007085117. Epub 2020 Jun 8. 10.1073/pnas.2007085117 PubMed 32513738
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