pMM95
(Plasmid
#176615)
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PurposeExtracellular domain truncated. Used to test SIRPA exclusion from the phagocytic synpase.
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 176615 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHR
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFRBext-GFPint
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SpeciesSynthetic
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Mutationn/a
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMM95 was a gift from Meghan Morrissey & Ron Vale (Addgene plasmid # 176615 ; http://n2t.net/addgene:176615 ; RRID:Addgene_176615) -
For your References section:
CD47 Ligation Repositions the Inhibitory Receptor SIRPA to Suppress Integrin Activation and Phagocytosis. Morrissey MA, Kern N, Vale RD. Immunity. 2020 Aug 18;53(2):290-302.e6. doi: 10.1016/j.immuni.2020.07.008. Epub 2020 Aug 7. 10.1016/j.immuni.2020.07.008 PubMed 32768386