GFP-RRN3/TIF1A
(Plasmid #17661)

• Depositing Lab: Tom Misteli
• Publication: Dundr et al Science. 2002 Nov 22. 298(5598):1623-6.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4730
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RRN3/TIF1A
• Alt name: Rrn3
• Alt name: TIF-IA
• Species: H. sapiens (human)
• Insert Size (bp): 1953
• Mutation: See depositor comments below
• Entrez Gene: RRN3 (a.k.a. A-270G1.2, TIFIA)
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: EGFP-C

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note: This plasmid contains a F236L mutation in RRN3 compared to the NCBI reference sequence. It is not known how this mutation affects plasmid function.