NK66
(Plasmid
#176606)
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PurposeTo measure Syk intensity in response to ligand clustering using DNA origami
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 176606 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHR
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSyk-BFP
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SpeciesM. musculus (mouse), Synthetic
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Mutationn/a
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Entrez GeneSyk (a.k.a. Sykb)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
NK66 was a gift from Meghan Morrissey (Addgene plasmid # 176606 ; http://n2t.net/addgene:176606 ; RRID:Addgene_176606) -
For your References section:
Tight nanoscale clustering of Fcgamma receptors using DNA origami promotes phagocytosis. Kern N, Dong R, Douglas SM, Vale RD, Morrissey MA. Elife. 2021 Jun 3;10. pii: 68311. doi: 10.7554/eLife.68311. 10.7554/eLife.68311 PubMed 34080973