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Addgene

IBAR-Cry2PHR
(Plasmid #176604)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 176604 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pmCherry-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4722
  • Total vector size (bp) 6979
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    IBAR-Cry2PHR-mCherry
  • Species
    H. sapiens (human), A. thaliana (mustard weed)
  • Insert Size (bp)
    2988
  • GenBank ID
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site nheI (not destroyed)
  • 3′ cloning site noti (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    IBAR-Cry2PHR was a gift from Robert Hughes (Addgene plasmid # 176604 ; http://n2t.net/addgene:176604 ; RRID:Addgene_176604)

  • For your References section:

    CRY-BARs: Versatile light-gated molecular tools for the remodeling of membrane architectures. Wurz AI, Bunner WP, Szatmari EM, Hughes RM. J Biol Chem. 2022 Aug 18;298(10):102388. doi: 10.1016/j.jbc.2022.102388. 10.1016/j.jbc.2022.102388 PubMed 35987384
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