pAAV_K700E_hSF3B1
(Plasmid
#176591)
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PurposeAdenoviral construct to change K to E at 700th amino acid (K700E) in human SF3B1
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 176591 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepAAV_SEPT
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSF3B1
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SpeciesH. sapiens (human)
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Entrez GeneSF3B1 (a.k.a. Hsh155, MDS, PRP10, PRPF10, SAP155, SF3b155)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV_K700E_hSF3B1 was a gift from Manoj Pillai (Addgene plasmid # 176591 ; http://n2t.net/addgene:176591 ; RRID:Addgene_176591) -
For your References section:
Generation of scalable cancer models by combining AAV-intron-trap, CRISPR/Cas9, and inducible Cre-recombinase. Boddu PC, Gupta AK, Kim JS, Neugebauer KM, Waldman T, Pillai MM. Commun Biol. 2021 Oct 13;4(1):1184. doi: 10.1038/s42003-021-02690-1. 10.1038/s42003-021-02690-1 PubMed 34645977
