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pHS0535 CRISPR-associated IscB (Chesapeake Bay) in pBR322 with Fn spacer
(Plasmid #176589)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 176589 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBR322
  • Backbone manufacturer
    NEB
  • Backbone size w/o insert (bp) 4361
  • Total vector size (bp) 7178
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    CRISPR-associate IscB (Chesapeake Bay) locus
  • Alt name
    IscB(HEARO)
  • Species
    Chesapeake Bay metagenome
  • Insert Size (bp)
    2817
  • Mutation
    Truncated CRISPR array to two direct repeats, replaced spacer in CRISPR array with Fn spacer

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHS0535 CRISPR-associated IscB (Chesapeake Bay) in pBR322 with Fn spacer was a gift from Feng Zhang (Addgene plasmid # 176589 ; http://n2t.net/addgene:176589 ; RRID:Addgene_176589)

  • For your References section:

    The widespread IS200/IS605 transposon family encodes diverse programmable RNA-guided endonucleases. Altae-Tran H, Kannan S, Demircioglu FE, Oshiro R, Nety SP, McKay LJ, Dlakic M, Inskeep WP, Makarova KS, Macrae RK, Koonin EV, Zhang F. Science. 2021 Oct;374(6563):57-65. doi: 10.1126/science.abj6856. Epub 2021 Sep 9. 10.1126/science.abj6856 PubMed 34591643
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