pHS0537 CRISPR-associated IscB locus (Delaware Bay) in pBR322 with Fn spacer
(Plasmid
#176588)
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PurposeEndogenous locus of CRISPR-associated IscB (Delaware Bay) with Fn spacer
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 176588 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBR322
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Backbone manufacturerNEB
- Backbone size w/o insert (bp) 4361
- Total vector size (bp) 8010
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameCRISPR-associate IscB (Delaware Bay) locus
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Alt nameIscB(HEARO)
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SpeciesDelaware Bay metagenome
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Insert Size (bp)3649
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MutationReplaced spacer in CRISPR array with Fn spacer
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHS0537 CRISPR-associated IscB locus (Delaware Bay) in pBR322 with Fn spacer was a gift from Feng Zhang (Addgene plasmid # 176588 ; http://n2t.net/addgene:176588 ; RRID:Addgene_176588) -
For your References section:
The widespread IS200/IS605 transposon family encodes diverse programmable RNA-guided endonucleases. Altae-Tran H, Kannan S, Demircioglu FE, Oshiro R, Nety SP, McKay LJ, Dlakic M, Inskeep WP, Makarova KS, Macrae RK, Koonin EV, Zhang F. Science. 2021 Oct;374(6563):57-65. doi: 10.1126/science.abj6856. Epub 2021 Sep 9. 10.1126/science.abj6856 PubMed 34591643
